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Thursday, 3 November 2016
Highlighted Article: LC-MS for Determination of Three Methoxyflavones from Kaempferia parviflora in rat Plasma and Application to Pharmacokinetic Study
LC-MS for Determination of Three Methoxyflavones from Kaempferia parviflora in rat Plasma and Application to Pharmacokinetic Study
Author(s):
Sarunya Tuntiyasawasdikul and Bungorn Sripanidkulchai Pages 371 - 378 (8)
Abstract:
Background: Methoxyflavones are isolated from Kaempferia parviflora, a Thai herb used in traditional medicine. The method of quantification of low level of methoxyflavones in biological samples needs to be optimized.
Objectives: To develop and validate a sensitive liquid chromatography-tandem mass spectrometry (LCMS) for the determination of methoxyflavones in rat plasma and apply for pharmacokinetic study.
Method: Chromatographic separation was achieved on a reverse phase C18 column, using acetonitrile and 0.5% formic acid in water in a ratio of 10:90 v/v to 50:50 v/v as the mobile phase, and a flow-rate of 0.2 ml/min at 25°C. LC–MS detection was performed under positive ionization with selective ion monitoring using target ions.
Results: The calibration curve showed good linearity in the concentration range of 10–3000 ng/ml. The limits of detection (LOD) and quantification (LOQ) were 0.43-0.78 ng/ml and 0.93-2.35 ng/ml, respectively. The intra-day and the inter-day variances were less than 6.41% and 12.96%. The mean recovery of methoxyflavones was 73.73- 99.76%. The assay was successfully applied to a pharmacokinetics study in rats that used a topical application of 15% K. parviflora extract in IPM-based vehicle. After topical administration, the plasma concentration of methoxyflavones continuously increased up to 48 h, indicating prolonged absorption of methoxyflavones into systemic circulation, with the maximum concentration (Cmax) of methoxyflavones being 401.94 ng/ml at 1 h.
Conclusion: The current method has been successfully applied to the determination of methoxyflavones in rat plasma and was used to study its pharmacokinetics under topical administration.
Keywords:
Kaempferia parviflora, methoxyflavones, LC-MS, pharmacokinetics, topical, validation.
Affiliation:
Center for Research and Development of Herbal Health Products, Khon Kaen University, Khon Kaen, 40002 Thailand.
Graphical Abstract:
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Wednesday, 26 October 2016
Most Accessed Article: A Novel Application of Three Phase Hollow Fiber Based Liquid Phase Microextraction (HF-LPME) for the HPLC Determination of Tamsulosin from Biological Fluids
A Novel Application of
Three Phase Hollow Fiber Based Liquid Phase Microextraction (HF-LPME) for the
HPLC Determination of Tamsulosin from Biological Fluids
Author(s):
Roghayeh A. Afshari and Mahnaz QomiPages
258-265 (8)
Abstract:
Background: Tamsulosin is used for the treatment of difficult urination, a common symptom of an enlarged prostate.
Objective: In the present paper, for the first
time a microextraction technique was introduced for the detection and
determination of tamsulosin in urine and plasma samples.
Method: Procedure for extraction of tamsulosin
consists of hollow ber based liquid phase microextraction (HF-LPME) followed by
high performance liquid chromatography (HPLC) coupled with ultraviolet (UV)
detection. The organic liquid membrane consists of 1-Octanol immobilized in the
pores of a hollow fiber. A pH gradient was applied to migrate analytes from the
sample solution with pH 10.5, through the organic liquid membrane into an
acidic acceptor solution with pH 3.0 which was located inside the lumen of
hollow fiber.
Results: Extraction recoveries greater than
80% were obtained in different biological matrices which resulted in
preconcentration factors greater than 102 and acceptable repeatability (2.5
< RSD% <3.5).
Conclusion: The method offers good linearity
with estimation of coefficacy higher than 0.9990. Finally, it was applied for
the determination and quantification of tamsulosin in human plasma and urine
samples.
Keywords:
Tamsulosin, high performance liquid
chromatography, hollow ber based liquid phase microextraction, Microextraction.
Affiliation:
Department of medicinal chemistry,
pharmaceutical sciences research center, University of Pharmaceutical sciences
branch Islamic Azad Tehran, Iran.
Graphical Abstract:
