A Novel Application of
Three Phase Hollow Fiber Based Liquid Phase Microextraction (HF-LPME) for the
HPLC Determination of Tamsulosin from Biological Fluids
Author(s):
Roghayeh A. Afshari and Mahnaz QomiPages
258-265 (8)
Abstract:
Background: Tamsulosin is used for the treatment of difficult urination, a common symptom of an enlarged prostate.
Objective: In the present paper, for the first
time a microextraction technique was introduced for the detection and
determination of tamsulosin in urine and plasma samples.
Method: Procedure for extraction of tamsulosin
consists of hollow ber based liquid phase microextraction (HF-LPME) followed by
high performance liquid chromatography (HPLC) coupled with ultraviolet (UV)
detection. The organic liquid membrane consists of 1-Octanol immobilized in the
pores of a hollow fiber. A pH gradient was applied to migrate analytes from the
sample solution with pH 10.5, through the organic liquid membrane into an
acidic acceptor solution with pH 3.0 which was located inside the lumen of
hollow fiber.
Results: Extraction recoveries greater than
80% were obtained in different biological matrices which resulted in
preconcentration factors greater than 102 and acceptable repeatability (2.5
< RSD% <3.5).
Conclusion: The method offers good linearity
with estimation of coefficacy higher than 0.9990. Finally, it was applied for
the determination and quantification of tamsulosin in human plasma and urine
samples.
Keywords:
Tamsulosin, high performance liquid
chromatography, hollow ber based liquid phase microextraction, Microextraction.
Affiliation:
Department of medicinal chemistry,
pharmaceutical sciences research center, University of Pharmaceutical sciences
branch Islamic Azad Tehran, Iran.
Graphical Abstract:
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